EPI018
Sirtuin Activity Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Sinonimo/i:
Activity Assay for Sirtuins, Fluorometric Sirtuin Kit, Sirtuin Assay Kit
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.84
Prodotti consigliati
impiego
sufficient for 100 fluorometric tests
Metodo di rivelazione
fluorometric
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Descrizione generale
Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Compatibilità
Suitable for the detection of Sirtuin Activity in purified recombinant protein, cell and tissue lysate, nuclear extract, mitochondria and immunoprecipitated samples
Principio
In the Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically ((λex = 400/λem = 505 nm). HDAC′s also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC′s in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay
is 0.06 μU of recombinant human SIRT6.
is 0.06 μU of recombinant human SIRT6.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Dam. 1 - Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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