A white polystyrene plate with a non-binding surface is recommended for this kit. Black plates have not been evaluated, but may have a lower signal. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/329/297/cs1040bul.pdf
CS1040
SIRT1 Assay Kit
sufficient for 100 assays
Sinonimo/i:
SIRT1 Activity Assay
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 kit | Per conoscere la disponibilità, visualizza il carrello | CHF 695.00 |
Informazioni su questo articolo
NACRES:
NA.84
UNSPSC Code:
12161503
CHF 695.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartiusage
sufficient for 100 assays
Quality Segment
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... SIRT1(23411)
General description
The SIRT1 Assay Kit offers all the reagents required for the measurement of purified SIRT1 activity and for screening of inhibitors/activators.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
Application
SIRT1 Assay Kit has been used in measuring the Sirt1 deacetylase activity in mesenchymal stem cells (MSCs), human retinal endothelial cells (HRECs) and human neuroblastoma SH-SY5Y cells.
Biochem/physiol Actions
Sirtuins (Sir2) are an evolutionarily conserved family of NAD+ dependent histone/protein deacetylases that tightly couple the cleavage of NAD+ and the deacetylation of protein substrates. The reaction products are nicotinamide, the deacetylated product, and a novel metabolite, 2′-O-acetyl-ADP-ribose. The proteins within this family are named after the first protein discovered from this family, Sir2 (Silent Information Regulator 2). Besides gene silencing, sirtuin proteins are important in other processes such as cell cycling regulation and fatty acid metabolism. SIRT1 is the human homolog of Sir2 and the one most studied to date. SIRT1 mediates p53 dependent process, transcription regulation, muscle differentiation, adipogenesis, and protection from axonal degeneration. SIRT1 also participates in early embryogenesis, neurogenesis, and cardiogenesis.
Features and Benefits
The kit offers all the reagents required for the fast and easy measurement of purified SIRT1 activity and for screening of inhibitors/activators. Moreover, the kit contains an inhibitor (nicotinamide) and an activator (resveratrol) as negative and positive controls, respectively.
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Questo articolo | |||
|---|---|---|---|
| description sufficient for 100 assays | description 1 kit sufficient for 100 assays (1 mL) | description recombinant, expressed in E. coli, N-terminal histidine tagged, ≥90% (SDS-PAGE), buffered aqueous glycerol solution | description Upstate®, from rabbit |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| usage sufficient for 100 assays | usage kit sufficient for 100 assays (1 mL) | usage - | usage - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Gene Information human ... SIRT1(23411) | Gene Information human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112) | Gene Information human ... SIRT1(23411) | Gene Information human ... SIRT1(23411) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS & Determinazione del prezzo
- SIRT1 human, recombinant, expressed in E. coli, N-terminal histidine tagged, ≥90% (SDS-PAGE), buffered aqueous glycerol solution 150 μg
Classe di stoccaggio
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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Can I use black flat bottom plates with polystyrene, nonbonding surface in this measurment?
1 answer-
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CAN I USE THE SIRT1 ACTIVITY ASSAY KIT CS1040 TO ESTIMATE SIRT1 ENZYMATIC ACTIVITY IN MICE LUNG TISSUE LYSATE?
1 answer-
The kit is intended to measure purified SIRT1 activity and to screen the efficacy of inhibitors and activators. However, it has also been cited to assess SIRT1 enzymatic activity in cell lysates, including mice tissue lysates. The following citation may be of interest for reference:
Heliyon. 2024 Sep 4;10(17):e37083.
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