Unfortunately, there is no direct correlation between linear pore size measurements and nominal pore ratings based on retention of globular proteins (MWCO). However, several publications are available describing the separation of QDs by size based on the membrane MWCO. Please see the link below to review an example of one such publication:
https://pmc.ncbi.nlm.nih.gov/articles/PMC8271461/
D9652
Dialysis tubing cellulose membrane
avg. flat width 33 mm (1.3 in.)
Sinonimo/i:
Cellulose Dialysis Tubing, Cellulose Membrane, Dialysis Membrane
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About This Item
Codice UNSPSC:
41123100
NACRES:
NB.22
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Diametro medio
21 mm , when full
Larg.media se piatto
33 mm (1.3 in.)
Capacità
~110 mL/ft
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Descrizione generale
Typical molecular weight cut-off = 14,000.
Confezionamento
Supplied in rolls, dry
Qualità
May contain glycerin and sulfur compounds in more than trace amounts.
Nota sulla preparazione
Removal of glycerol included as a humectant can be accomplished by washing the tubing in running water for 3-4 hours. Removal of sulfur compounds can be accomplished by treating the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute. Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid. This tubing will retain most proteins of molecular weight 12,000 or greater.
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Lot/Batch Number
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Contenuto correlato
Dialysis Tubing
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What is the molecular weight cut off for the Dialysis tubing cellulose membrane avg. flat width 33 mm (1.3 in.) with product number D9652? Is this suitable for purification of Quantum Dots which have size range from 2-10nm.
1 answer-
Helpful?
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Which organic solvents can be used with these membranes?
1 answer-
Regenerated cellulose membranes are typically compatible with acids and alcohols. Organic solvent compatibility varies from compound to compound. However, the solvent concentration, temperature, and exposure time are factors to be considered. Please see the table below to review the general membrane compatibility with various solvents.
Helpful?
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how do I prepare the tubings before usage?
1 answer-
Instructions have been provided in the 'Preparation Note' in the 'DESCRIPTION' section.
-Wash the tubing in running water for 3-4 hours to remove glycerol included as a humectant.
-Treat the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute to remove sulfur compounds.
-Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid.
This tubing will retain most proteins of molecular weight 12,000 or greater.Helpful?
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