The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate. Historical data also note that this material may be reconstituted in PBS or other physiologic buffers containing Ca2+, culture medium, or water; however, no concentration is provided.
D4818
Dispase I
protease
Sinonimo/i:
Proteasi
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Cambia visualizzazione
2 MG
CHF 257.00
About This Item
Prodotti consigliati
Livello qualitativo
Stato
lyophilized solid
Attività specifica
≥10 unit/mg solid
tecniche
cell culture | mammalian: suitable
single cell analysis: suitable
Temperatura di conservazione
2-8°C
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Applicazioni
Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.
Dispase I has been used in lung digestion and processing for flow staining, as well as for CD4 cell isolation in mice.[1] The enzyme has also been used to digest excised wounds and a small amount of surrounding skin for the detection of GFP+ (green fluorescence protein) cells. This study to investigated the effect of differentiation and angiogenesis of bone marrow-derived mesenchymal stem cells on wound healing.[2] It has also been used to remove the epidermis during the isolation of dermal fibroblasts from mice.[3]
Suitable for use in preparation of single cell suspension for sequencing.
Suitable for use in preparation of single cell suspension for sequencing.
Azioni biochim/fisiol
Dispase I is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. It can also separate intact epithelial sheets in culture from the substratum. The enzyme preserves the viability of the epithelial cells while cleaving the basement membrane zone region. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] It hydrolyzes N-terminal peptide bonds of non-polar amino acid residues. It preferentially attacks denatured and intercellular proteins with exposed hydrophobic amino acid residues. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[5] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.
Definizione di unità
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Stato fisico
lyophilized powder containing calcium acetate
Note legali
Dispase is a registered trademark of Godo Shusei Co., Ltd.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Certificati d'analisi (COA)
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I clienti hanno visto anche
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Yaojiong Wu et al.
Stem cells (Dayton, Ohio), 25(10), 2648-2659 (2007-07-07)
Although chronic wounds are common, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefit of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing. Using an excisional wound splinting model, we showed
Liwen Chen et al.
PloS one, 4(9), e7119-e7119 (2009-09-23)
Studies have shown that allogeneic (allo-) bone marrow derived mesenchymal stem cells (BM-MSCs) may enhance tissue repair/regeneration. However, recent studies suggest that immune rejection may occur to allo-MSCs leading to reduced engraftment. In this study, we compared allo-BM-MSCs with syngeneic
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P J Griffin et al.
The Biochemical journal, 125(4), 109P-109P (1971-12-01)
Articoli
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Contenuto correlato
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
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How to disolve dispase? Can I use mediums as DMEM, PBS ...?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of Product D4818, Dispase I?
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The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate.
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What is the difference between Product D4693, Dispase II and Product D4818, Dispase I?
1 answer-
Product D4693 is a lyophilized powder containing calcium acetate and milk sugar as a stabilizer. The activity specification is >0.5 unit/mg solid. Product D4818 does not contain any milk sugar and has an activity specification of >10 units/mg solid.
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What is the activity of the Product D4818, Dispase, which I received?
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Each lot will have a specific activity that can be found on our website on the certificate of analysis.
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At what concentration should Product D4818, Dispase, be used?
1 answer-
To dissociate cells from ECM (extracellular matrix) use at a concentration of 0.6 - 2.4 units/ml.
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