MCF7/LetR-1

16022524, human breast, Polygonal epithelia

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: MCF7/LetR-1, from human breast, 16022524
• Origine biologica: human breast
• Modalità di accrescimento: Adherent
• Morfologia: Polygonal epithelia
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

This breast cancer cell line resistant to the aromatase inhibitor letrozole. Oestrogen receptor positive. It is progesterone receptor positive when grown in medium without letrozole

Descrizione della linea cellulare

The MCF7/LetR-1 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors.
Letrozole-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10^-7 M testosterone. A culture of MCF-7 cells were treated with 10^-6 M letrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with letrozole. After ~2–3 months, the isolated colonies gave rise to letrozole-resistant cell lines, which could be grown in letrozole-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.

Terreno di coltura

Phenol red free DMEM:F12 (1:1) (D6434) + 10% Newborn Calf Serum(NBCS) (N4637) + 2.5 mM Ala-Gln (G8541) + 6 ng/ ml Insulin (I9278), 0.1 uM testosterone and 1 uM letrozole

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.05% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citazione

If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7/LetR-1 (ECACC 16022524)

Esclusione di responsabilità

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.