C3787

Chloramphenicol succinate sodium salt

≥80% (HPLC)

• Sinonimo/i: Chloramphenicol α-succinate
• Formula condensata: C₁₅H₁₅Cl₂N₂O₈Na
• Numero CAS: 982-57-0
• Peso molecolare: 445.18
• Numero CE: 213-568-1
• Numero MDL: MFCD00079025
• Codice UNSPSC: 41116107
• ID PubChem: 24892643
• NACRES: NA.85

Proprietà

• Saggio: ≥80% (HPLC)
• Forma fisica: solid
• Solubilità: H₂O: 50 mg/mL
• Spettro attività antibiotica: Gram-negative bacteria; Gram-positive bacteria
• Modalità d’azione: protein synthesis | interferes
• Stringa SMILE: [Na].O[C@@H]([C@@H](COC(=O)CCC(O)=O)NC(=O)C(Cl)Cl)c1ccc(cc1)N(=O)=O
• InChI: 1S/C15H16Cl2N2O8.Na.H/c16-14(17)15(24)18-10(7-27-12(22)6-5-11(20)21)13(23)8-1-3-9(4-2-8)19(25)26;;/h1-4,10,13-14,23H,5-7H2,(H,18,24)(H,20,21);;/t10-,13-;;/m1../s1; RJOAHMNSYANTPN-OWVUFADGSA-N

Descrizione

Descrizione generale

Chemical structure: phenicole

Applicazioni

Chloramphenicol is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

Azioni biochim/fisiol

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Avvertenza

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

Nota sulla preparazione

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

Altre note

Keep container tightly closed in a dry and well-ventilated place.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302,H351
• Consigli di prudenza: P202 - P264 - P280 - P301 + P312 - P308 + P313 - P405
• Classi di pericolo: Acute Tox. 4 Oral - Carc. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Dispositivi di protezione individuale: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges