The activity of this enzyme is established using a 0.02 M Tris buffer with a starting pH of 8.3 at 0°C. One unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. However, the optimum pH and temperature is highly dependent on the specific application. There are a number of publications regarding the working parameters for this material. Please see the link below to review an example:
https://pubmed.ncbi.nlm.nih.gov/10701452/#:~:text=It%20was%20determined%20that%20the,61706%20Dalton%20by%20gel%20filtration.
C2624
Anidrasi carbonica
≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder
Sinonimo/i:
Carbonato deidratasi, Carbonato idrolasi
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100 MG
CHF 319.00
500 MG
CHF 1’180.00
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Cambia visualizzazione
100 MG
CHF 319.00
500 MG
CHF 1’180.00
About This Item
Prodotti consigliati
Origine biologica
bovine erythrocytes
Saggio
≥75% protein basis (biuret)
≥95% (SDS-PAGE)
Stato
lyophilized powder
Attività specifica
≥3,500 W-A units/mg protein
Solubilità
deionized water: >10 mg/mL
Temperatura di conservazione
2-8°C
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Applicazioni
Azioni biochim/fisiol
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications [3].
Definizione di unità
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Carbonic anhydrase type II deficiency syndrome is an uncommon autosomal recessive disease with cardinal features including osteopetrosis, renal tubular acidosis and brain calcifications. We describe the neurological, neuro-ophthalmological and neuroradiological features of 23 individuals (10 males, 13 females; ages at
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What is recommand usage temperature and pH range?
1 answer-
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What is the recommended storage conditions for "Carbonic Anhydrase, from bovine erythrocytes?
1 answer-
The recommended storage conditions for Solution of Carbonic Anhydrase, from bovine erythrocytes may be frozen at –20 °C for future use. It is advised to avoid repeated freezing and thawing. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2–3 uses.
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What is the isozyme I or isozyme II of Carbonic Anhydrase from bovine erythrocytes (C2624 Carbonic Anhydrase)?
1 answer-
The product C2624, Carbonic Anhydrase from bovine erythrocytes, consists mainly of CA II. However, there are also small levels of CA I and CA IV present.
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Is it possible to obtain information about the type of Carbonic Anhydrase (I or II) included in item C2624-carbonic anhydrase?
1 answer-
Product C2624, Carbonic Anhydrase from bovine erythrocytes, with a purity of ≥95% (SDS-PAGE), consists predominantly of CA II, with small levels of CA I and CA IV present.
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