C2522

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥3,000 W-A units/mg protein

• Sinonimo/i: Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
• Numero CAS: 9001-03-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-576-6
• MDL number: MFCD00081467
• Numero CE: 4.2.1.1 (BRENDA, IUBMB)
• Specific activity: ≥2475 units/mg solid, ≥3,000 W-A units/mg protein
• Biological source: bovine erythrocytes

Proprietà

• biological source: bovine erythrocytes
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥2475 units/mg solid, ≥3,000 W-A units/mg protein
• mol wt: 30 kDa
• pI: ~5.9
• storage temp.: −20°C

Descrizione

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD₄⁺ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Parola di avvertenza: Danger
• Codici di pericolo: H334
• Avvertenze di sicurezza: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe di stoccaggio: 11 - Combustible Solids
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): Eyeshields, Gloves, type N95 (US)