B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Sinonimo/i: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
• Formula empirica (notazione di Hill): C₆H₁₅NO₅S
• Numero CAS: 10191-18-1
• Peso molecolare: 213.25
• Beilstein: 1781572
• Numero CE: 233-465-5
• Numero MDL: MFCD00007533
• Codice UNSPSC: 12161700
• ID PubChem: 24891788
• NACRES: NA.25

Proprietà

• Grado: BioPerformance Certified
• Saggio: ≥99.0%
• Stato: crystalline
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin and bioburden, tested
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• Intervallo di pH utile: 6.4-7.8
• pKa (25 °C): 7.1
• Solubilità: water: 0.5 g/mL, clear, colorless
• applicazioni: diagnostic assay manufacturing
• Attività estranea: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• Temperatura di conservazione: room temp
• Stringa SMILE: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12); AJTVSSFTXWNIRG-UHFFFAOYSA-N

Descrizione

Descrizione generale

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Applicazioni

BES buffer has been used:

• as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
• as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
• as a component of diluent one for semen collection and cryopreservation

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves