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E9502

Sigma-Aldrich

EPPS

≥99.5% (titration)

Sinonimo/i:

Acido N-(2-idrossietil)piperazin-N′-(3-propansolfonico), Acido 4-(2-idrossietil)-1-piperazinpropansolfonico, Acido 4-(2-idrossietil)piperazin-1-propansolfonico, HEPPS

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About This Item

Formula empirica (notazione di Hill):
C9H20N2O4S
Numero CAS:
16052-06-5
Peso molecolare:
252.33
Beilstein:
3957385
Numero CE:
240-198-8
Numero MDL:
MFCD00006160
Codice UNSPSC:
12161700
ID PubChem:
24894698
NACRES:
NA.25

Livello qualitativo

400

Saggio

≥99.5% (titration)

Stato

powder

pH

7.3-8.7

Intervallo di pH utile

7.3-8.7

pKa (25 °C)

8.0

pKa 

8

Punto di fusione

237-239 °C (lit.)

Solubilità

H2O: 25 g + 50 mL, clear, colorless

λmax

A290 at ≤0.1 (C=33% w/w)

applicazioni

diagnostic assay manufacturing

Stringa SMILE

OCCN1CCN(CCCS(O)(=O)=O)CC1

InChI

1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)
OWXMKDGYPWMGEB-UHFFFAOYSA-N

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Categorie correlate

Applicazioni

EPPS has been used:
  • to verify the effect of pH variation on the proteolytic activity of the posterior midgut (PMG) contents and anterior midgut (AMG) tissue homogenate
  • to prepare a destaining buffer to destain intensely stained gel pieces
  • to prepare digestion buffer for in-gel enzymatic digestion
  • to reconstitute the digested peptides for the processing of brain-derived extracellular vesicle (EV) proteins and 16-plex isobaric tandem mass tag (TMT) peptide labeling

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000409395
0000409395
0000401816
0000401818
0000401814

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CHES ≥99.0% (titration)

Sigma-Aldrich

C2885

CHES

HEPES ≥99.5% (titration)

Sigma-Aldrich

H3375

HEPES

Bernhard Sissolak et al.
Journal of industrial microbiology & biotechnology, 46(8), 1167-1178 (2019-06-09)
Recombinant monoclonal antibodies are predominantly produced in mammalian cell culture bioprocesses. Post-translational modifications affect the micro-heterogeneity of the product and thereby influence important quality attributes, such as stability, solubility, pharmacodynamics and pharmacokinetics. The analysis of the surface charge distribution of
YoungSoo Kim et al.
Science advances, 5(4), eaav1388-eaav1388 (2019-04-20)
Detection of amyloid-β (Aβ) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Aβ is deemed a less invasive and more accessible hallmark of AD, as Aβ can penetrate blood-brain barriers. However, correlations between biofluidic Aβ concentrations and AD
Bianca Santos Henriques et al.
Frontiers in physiology, 11, 509310-509310 (2021-02-02)
Rhodnius prolixus is one important vector for the parasite Trypanosoma cruzi in Latin America, where Chagas disease is a significant health issue. Although R. prolixus is a model for investigations of vector-parasite interaction and transmission, not much has been done
Heeseon An et al.
Nature, 583(7815), 303-309 (2020-07-03)
Mammalian cells reorganize their proteomes in response to nutrient stress through translational suppression and degradative mechanisms using the proteasome and autophagy systems1,2. Ribosomes are central targets of this response, as they are responsible for translation and subject to lysosomal turnover
Yang You et al.
Journal of extracellular vesicles, 11(1), e12183-e12183 (2022-01-15)
In neurodegenerative diseases, extracellular vesicles (EVs) transfer pathogenic molecules and are consequently involved in disease progression. We have investigated the proteomic profiles of EVs that were isolated from four different human-induced pluripotent stem cell-derived neural cell types (excitatory neurons, astrocytes
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