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B9754

BIS-TRIS

≥98.0% (titration)

Sinonimo/i:

2,2-bis(idrossimetil)-2,2′,2″-nitrilotrietanolo, 2-bis(2-idrossietil)ammino-2-(idrossimetil)-1,3-propandiolo, Bis(2-idrossietil)ammino-tris(idrossimetil)metano

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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C8H19NO5
Numero CAS:
Peso molecolare:
209.24
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
230-237-7
Beilstein/REAXYS Number:
2205275
MDL number:
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Quality Segment

assay

≥98.0% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

color

white

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

suitability

suitable for Western blot, suitable for column chromatography of proteins

application(s)

advanced drug delivery
diagnostic assay manufacturing
general analytical
microbiology

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

General description

Il bis-tris metano, noto anche come BIS-TRIS o BTM, è un agente tamponante zwitterionico utilizzato diffusamente nella ricerca biochimica e biologica. Agisce in maniera efficace in soluzione acquosa in un intervallo di pH che va da 5,8 a 7,2 a valori che si discostano in misura minima dalla sua costante di dissociazione. Inoltre, può essere impiegato come sostituto del tampone cacodilato, altamente tossico, così come è noto per la sua capacità di formare complessi con vari metalli.

Application

Il Bis-Tris è stato usato:
  • come tampone per elettroforesi
  • come componente di un tampone di trasferimento per il western blotting

Features and Benefits

  • Indicato per la ricerca in biologia e in biochimica
  • Tampone efficace nell′intervallo di pH 5,8-7,2 (25 °C), con una pKa di 6,5 (25 °C)

Other Notes

Per ulteriori informazioni sulla nostra gamma di Composti biochimici, compilare questo modulo.

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BIS-TRIS ≥98.0% (titration)

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BIS-TRIS anhydrous, free-flowing, Redi-Dri™, ≥98.0%

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BIS-TRIS

BIS-TRIS BioXtra, ≥98.0% (titration)

Sigma-Aldrich

B7535

BIS-TRIS

description

≥98.0% (titration)

description

BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

description

anhydrous, free-flowing, Redi-Dri, ≥98.0%

description

BioXtra, ≥98.0% (titration)

assay

≥98.0% (titration)

assay

≥98.0%

assay

≥98.0%

assay

≥98.0% (titration)

technique(s)

electrophoresis: suitable, UV/Vis spectroscopy: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

100

Quality Level

400

form

crystalline powder

form

crystalline powder

form

crystalline powder

form

powder

application(s)

advanced drug delivery
diagnostic assay manufacturing
general analytical
microbiology

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

water: 500 mg/mL, clear, colorless to faint yellow or tan

solubility

H2O: 1 M, clear, colorless


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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