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Merck
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A8312

Sigma-Aldrich

m-Aminophenylboronic acid–Agarose

aqueous suspension

Sinonimo/i:

(3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin

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About This Item

Numero MDL:
MFCD00801303
Codice UNSPSC:
12161501
ID PubChem:
329770955
NACRES:
NA.32

Forma fisica

aqueous suspension

Grado di funzionalizzazione

40-80 μmol per mL

Matrice

6% beaded agarose

Attivazione matrice

epichlorohydrin

Gruppi immobilizzati alla matrice

through amino to carboxyls of EDTA

Braccio spaziatore

9 atoms

Capacità

≥8 mg/mL binding capacity (peroxidase Type VI)

Temperatura di conservazione

2-8°C

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Descrizione generale

m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.

Applicazioni

m-Aminophenylboronic acid-Agarose has been used:
  • as a component of the column for the purification of Escherichia coli TOP10F cells
  • with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
  • in the affinity purification of horseradish peroxidase (HRPO)
  • in the glycated human serum albumin (gHSA) purification

Stato fisico

Suspension in water containing 0.002% chlorhexidine diacetate.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Construction and optimization of a CC49-Based scFv-beta-lactamase fusion protein for ADEPT
Roberge M, et al.
Protein engineering, design & selection : PEDS, 19(4), 141-145 (2006)
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
Monika Kijewska et al.
Molecules (Basel, Switzerland), 25(3) (2020-02-14)
We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of a lysine residue to specifically capture deoxyfructosylated peptides, compared to differently glycosylated peptides in complex mixtures. The
Pravin K Bhatnagar et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 235-241 (2008-02-09)
Hybrid hybridomas (quadromas) are derived by fusing at least two hybridomas, each producing a different antibody of predefined specificity. The resulting cell secretes not only the immunoglobulins of both parents but also hybrid molecules manifesting the binding characteristics of the
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