813066

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, m-aminophenyl boronate phase

• Sinonimo/i: TSKgel^® Boronate-5PW HPLC Column
• Codice UNSPSC: 41115700

Proprietà

• product name: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
• Materiali: PEEK
• Nome Commerciale: TSKgel^®
• Classi chimiche degli analiti: proteins
• Produttore/marchio commerciale: Tosoh Boronate-5PW, 13066
• Parametri: ≤20% organic solvent; ≤3 M salt concentration; 1.2 mL/min flow rate; 30 bar max. pressure; ≥ 0 °C temperature
• tecniche: HPLC: suitable
• Lungh. × D.I.: 7.5 cm × 7.5 mm
• Matrice: polymer particle platform
• Gruppo funzionale matrice: m-aminophenyl boronate phase
• Dimensione particelle: 10 μm
• Dimensione pori: 1000 Å
• pH di lavoro: 2-9
• Capacità: 40 μmol/mL adsorption capacity (sorbitol)
• Compatibilità: mode of use affinity HPLC
• Tecnica di separazione: affinity
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

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Note legali

TSKgel is a registered trademark of Tosoh Corporation

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable