A3352
Acyl-coenzyme A Synthetase from Pseudomonas sp.
≥2 units/mg protein
Sinonimo/i:
Acid: CoA ligase (AMP-forming)
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 unit | Per conoscere la disponibilità, visualizza il carrello | CHF 237.00 |
10 units | Per conoscere la disponibilità, visualizza il carrello | CHF 1’180.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-747-5
MDL number:
Specific activity:
≥2 units/mg protein
Servizio Tecnico
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Permettici di aiutartiGeneral description
Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.
Application
Acyl-coenzyme A Synthetase may be used to study fatty acid metabolism and lipid metabolism. It has been used to study its interaction with fatty acid transport proteins, which has been found to be involved in the efficient cellular uptake of long-chain fatty acids in adipocytes [1].
Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:
- as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
- in in vitro fatty acylation assays
- in the synthesis of (14C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (3H)Palmitate-CoA
Biochem/physiol Actions
Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells [1].
Acyl-coenzyme A (CoA) synthetase (ACS) enzyme catalyzes a two-step thioesterification reaction involving the conversion of free fatty acids (FAs) to CoA esters. The substrate for ACS varies from two carbon to 26 carbon FAs. It is involved in the activation of FAs for lipid metabolism and enables FA to participate in various cellular metabolic pathways.
Other Notes
One unit will form 1.0 μmole of AMP and oleoyl coenzyme A from ATP and oleate at pH 8.1 at 25 °C in the presence of Coenzyme A.
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Questo articolo | |||
|---|---|---|---|
| specific activity ≥2 units/mg protein | specific activity ≥3 units/mg protein | specific activity ≥3 units/mg protein | specific activity ≥100 units/mg solid |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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