A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Sinonimo/i: Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Formula empirica (notazione di Hill): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• Numero CAS: 96087-38-6
• Peso molecolare: 593.70
• Numero MDL: MFCD00210085
• Codice UNSPSC: 12352200
• ID PubChem: 24890604
• NACRES: NA.52

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Saggio: ≥95%
• Stato: film or powder
• Solubilità: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• Temperatura di conservazione: −20°C
• Stringa SMILE: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1; FFBLNTOMOSLSQM-AYEYRVMASA-N

Descrizione

Descrizione generale

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.[1]

Applicazioni

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization[2] to determine genomic relatedness.[3][4]

Azioni biochim/fisiol

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.[1]

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins.[5] Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Avvertenza

Light sensitive.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)