76546

Lipase, immobilized on Immobead 150

≥3000 U/g

• Sinonimo/i: Immobilized lipase, Lipase enzyme
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: fungus (Thermomyces lanuginosus)
• Livello qualitativo: 100
• Stato: powder (or beads)
• Attività specifica: ≥3000 U/g
• tecniche: analytical sample preparation: suitable
• Colore: white to off-white
• applicazioni: life science and biopharma
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Research area: Cell Signaling

Lipase from Thermomyces lanuginosus (TLL) is a basophilic, single-chain protein and thermostable enzyme. It belongs to the alpha/beta-hydrolase fold family. TLL is roughly spherical and is made up of a central seven-stranded β-parallel sheet with five interconnecting α-helices.[1]

Applicazioni

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus has been used:

• as a biocatalyst to study ultrasound-assisted transesterification of a blend of non-edible oils with methanol.
• as a catalyst in the synthesis of medium chain fatty acids (MCFA) and polyunsaturated fatty acids (PUFA) rich oils by enzymatic structuring of flax seed oil.
• for the resolution of chiral compounds and the transesterification production of biodiesel.
• to enhance the efficiency and selectivity of the enzymatic synthesis of ethyl-(R)-2-hydroxy-4-phenylbutyrate, (R)-HPB ester. [2]
• to facilitate a chemoenzymatic process for the continuous synthesis of (S)-7-((tert-Butyldiphenylsilyl)oxy)hept-1-yn-4-ol, a crucial intermediate for Eribulin synthesis, within a packed-bed reactor setup.[3]

Azioni biochim/fisiol

It participates in catalyzing the hydrolysis of triglycerides at the water-lipid interface and produces mono-, di-glycerides, and free fatty acids. Due to its versatility, this enzyme is applicable in various industries including, detergents, paper, leather, baking, fine chemicals, fuel, and biocatalysts.[1]

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Definizione di unità

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)