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Merck
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Documenti fondamentali

66540401

Sigma-Aldrich

BIONi028-A

Human iPS Cell Line

Sinonimo/i:

EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC

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About This Item

Codice UNSPSC:
41106509
Prezzi e disponibilità al momento non sono disponibili

Origine biologica

human adipose-derived MSC

Livello qualitativo

Metodo di riprogrammazione

episomal

Descrizione

age (20-24)

Produttore/marchio commerciale

EBiSC

Genere

male

Modalità di accrescimento

adherent (pluripotent)

tecniche

cell culture | stem cell: suitable

Malattie correlate

none

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Descrizione generale

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Origine della linea cellulare

Depositor
Bioneer

Descrizione della linea cellulare

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File

Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SOX2(+)TRA 1-81(+)SSEA-1(-)TDG1(+)POU5F1 (OCT-4)(+)NANOG(+)DMNT3B(+)GDF3(+)GABRB3(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34 (+)CD56(+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Linkage

Note: EAUA and CLIP must be completed before order fulfillment

Mantenimento delle subcolture

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

Note legali

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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