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Key Documents

41699

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR RED antibody produced in goat

for STED application

Sinonimo/i:

Abberior® STAR RED-Anti-Rabbit IgG antibody produced in goat

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Origine biologica

goat

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

rabbit

Concentrazione

~1 mg/mL

Fluorescenza

λex 638 nm; λem 655 nm in PBS, pH 7.4

Temperatura di conservazione

−20°C

Descrizione generale

Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto 647N, Alexa Fluor 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)

Caratteristiche e vantaggi

  • Unmatched, background free STED imaging cont
  • Verified in Abberior Instruments and Leica STED microscopes

Compatibilità

Designed and tested for fluorescent super-resolution microscopy

Risultati analitici

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Note legali

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH

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Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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