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760765

Sigma-Aldrich

Azide-fluor 488

≥90% (HPLC)

Sinonimo/i:

Rhodamine 110-azide

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1 MG
CHF 97.90
5 MG
CHF 370.00

CHF 97.90


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1 MG
CHF 97.90
5 MG
CHF 370.00

About This Item

Formula empirica (notazione di Hill):
C29H30N6O7
Peso molecolare:
574.58
Codice UNSPSC:
12352125
NACRES:
NA.22

CHF 97.90


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Saggio

≥90% (HPLC)

Stato

solid

Impiego in reazioni chimiche

reaction type: click chemistry

Temperatura di conservazione

−20°C

Applicazioni

Rhodamine 110 conjugated PEG azide. Can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.

Spectral Properties: Abs/Em = 501/525 nm

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Certificati d'analisi (COA)

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Two-step local functionalization of fluoropolymer Dyneon THV microfluidic materials by scanning electrochemical microscopy combined to click reaction.
Slim C, et al.
Electrochemical Communications, 60, 5-8 (2015)
Synthesis and Conjugation of Alkyne-Functional Hyperbranched Polyglycerols.
Moore E, et al.
Macromolecular Chemistry and Physics, 217(20), 2252-2261 (2016)
Chemical tools for studying the biological function of glycolipids.
Stocker BL & Timmer MS
Chembiochem, 14(10), 1164-1184 (2013)
Drawing in poly (e-caprolactone) fibers: tuning mechanics, fiber dimensions and surface-modification density
Kim SE, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine (2017)
Indole-induced Reversion of Intrinsic Multi-antibiotic Resistance in Lysobacter enzymogenes.
Han Y, et al.
Applied and Environmental Microbiology, AEM-00995 (2017)

Articoli

Explore the principles and applications of click chemistry in drug discovery, highlighting efficient reactions that streamline the synthesis of bioactive compounds.

Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. After resuspending the Rho-azide (#760765) in DMF, I observed a color change to fluorescent yellow, whereas reconstitution in PBS buffer retains its pink color. Could this color change potentially affect the results of the click-chemistry?

    1 answer
    1. This product is not tested for solubility. Thus, the color change of the solution from solvent to solvent has not been investigated. However, the polarity of a given solvent may impact the color of the resulting solution. The color change is very unlikely to be related to click chemistry as DMF lacks an appropriate function group.

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  4. After reconstituting product 760765, should it still be stored at -20 degrees Celsius?

    1 answer
    1. The solution stability of this product has not been determined. However, various sources suggest that this material may be stored for up to one month at -20°C or six months at -80°C in organic solvents such as DMSO or DMF. Solutions prepared in water are less stable and should be used within several hours. Note that this information has not been validated.

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  5. Hello, why is this labeled as fluor 488 when it is a Rhodamine 110 and not an Alexa Fluor 488 fluorophore?

    1 answer
    1. As listed on the product detail page, Rhodamine 110-azide is a synonym of Azide-fluor 488.

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