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N3013

Nile Red

Powder

Sinonimo/i:

9-(diethylamino)-5H-benzo[a]phenoxazin-5-one, 9-(diethylamino)benzo[a]phenoxazin-5-one, Nile Blue A Oxazone

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Cambia visualizzazione
A voi/SKUDisponibilitàPrezzo
100 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 258.00
1 g
Per conoscere la disponibilità, visualizza il carrello
CHF 1500.00

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C20H18N2O2
Numero CAS:
7385-67-3
Peso molecolare:
318.37
NACRES:
NA.47
PubChem Substance ID:
24897612
UNSPSC Code:
12171500
EC Number:
230-966-0
MDL number:
MFCD00011639
Beilstein/REAXYS Number:
279110

CHF 258.00

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Nome del prodotto

Nile Red, Technical grade

grade

technical grade

Quality Segment

200

form

powder

composition

Carbon: 70.0-77.3%

Nitrogen: 7.5-8.8%

technique(s)

titration: suitable

mp

203-205 °C (lit.)

solubility

methanol: 1 mg/mL

λmax

553 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

General description

Nile Red is a lipophilic fluorescent benzophenoxazone dye that is metachromatic and solvatochromic.[1] It is an uncharged hydrophobic molecule with the ability to stain and visualize lipid droplets in cells and tissues.[2] It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red.[3]

Application

Nile red is widely employed in studies related to lipid metabolism, lipid droplet dynamics, lipid accumulation in cells or tissues, and lipid-based drug delivery systems. Its other applications include:
  • identification of microplastics in routine analysis of biological samples.[1]
  • detection of lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells.[4]
  • it exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.

Biochem/physiol Actions

Nile Red is lipophilic in nature and exploits the hydrophobic properties of molecules such as lipids and plastics that fluoresce when excited with certain wavelengths, facilitating quantification and identification.[1]

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Questo articolo
N07661912372485
Nile Red Technical grade

Sigma-Aldrich

N3013

Nile Red

Nile Blue A certified by the BSC

Sigma-Aldrich

N0766

Nile Blue A

Nile Red BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

19123

Nile Red

Nile Red suitable for microscopy

Sigma-Aldrich

72485

Nile Red

technique(s)

titration: suitable

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

grade

technical grade

grade

-

grade

-

grade

-

solubility

methanol: 1 mg/mL

solubility

H2O: 1 mg/mL

solubility

-

solubility

methanol: 0.01 g/10 mL (red to very dark red)

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

form

powder

form

powder

form

solid

form

crystals

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

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Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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Questions

  1. How should n3013 be dissolved and diluted for lipid staining?

    1 answer

    1. There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
      To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.

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