PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• Sinonimo/i: 0.1M Tris
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Livello qualitativo: 100
• Nome Commerciale: pHast Pack^™
• Stato: powder
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: ≤5 ppm Heavy Metals (lead)
• Colore: white
• pH: 8.1
• Cationi in tracce: Fe: ≤10 ppm
• Attività estranea: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Applicazioni

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

Caratteristiche e vantaggi

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Confezionamento

Foil pouches

Ricostituzione

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Stoccaggio e stabilità

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1