This product is a mixture of human serum albumin (HSA) and human gamma-globulin, the latter of which is essentially all human IgG. The E1% (at 280 nm) values for HSA and human IgG are 14 and 5.3, respectively. The lot-specific extinction coefficient for the mixture can be calculated using the protein concentrations provided the certificate of analysis (CofA)._x000D_
Sample calculation, for Lot SLCN8399:_x000D_
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P8119-BULKSLCN8399.pdf_x000D_
The CofA reports an HSA concentration of 4.9 g/dL, and (by difference) a gamma-globulin concentration of 3.3 g/dL. The extinction coefficient at 280 nm for this 8.2 g/dL (82 mg/mL) lot is 86. Here is the calculation: (4.9 X 14) + (3.3 X 5.3) = 86.09, which would round to 86. If the standard were diluted by a factor of 8.2 to give a 1 g/dL or 1% solution, the E1% would be 10.5.
P8119
Protein Standard
analytical standard, 80 mg/mL (HSA and gamma-globulins)
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
Cambia visualizzazione
5 ML
CHF 64.20
About This Item
Codice UNSPSC:
85151701
NACRES:
NA.24
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Grado
analytical standard
Stato
liquid
contiene
0.05% sodium azide as preservative
Classi chimiche degli analiti
amino acids, peptides, proteins
Concentrazione
80 mg/mL (HSA and gamma-globulins)
tecniche
gel permeation chromatography (GPC): suitable
applicazioni
food and beverages
Formato
multi-component solution
Temperatura di conservazione
2-8°C
Categorie correlate
Applicazioni
Protein standard has been used in the determination of protein content of cod trypsin isoenzymes using bio-rad protein assay and ultraviolet-visible light spectrophotometry (UV-Vis).[1]
Altre note
Human serum albumin and gamma-globulins
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Bjarki Stefansson et al.
Biochimica et biophysica acta, 1865(1), 11-19 (2016-10-25)
Trypsins from Atlantic cod (Gadus morhua), consisting of several isoenzymes, are highly active cold-adapted serine proteases. These trypsins are isolated for biomedical use in an eco-friendly manner from underutilized seafood by-products. Our group has explored the biochemical properties of trypsins
Yu-Cheng Chen et al.
Optica, 3(8), 809-815 (2016-08-20)
Indocyanine green (ICG) is the only near-infrared dye approved by the U.S. Food and Drug Administration for clinical use. When injected in blood, ICG binds primarily to plasma proteins and lipoproteins, resulting in enhanced fluorescence. Recently, the optofluidic laser has
Elucidation of different cold-adapted Atlantic cod (Gadus morhua) trypsin X isoenzymes
Stefansson B, et al.
Biochimica et Biophysica Acta, 1865(1), 11-19 (2017)
Mehdi Alikhani et al.
Journal of proteomics, 162, 141-154 (2017-02-19)
Male infertility accounts for half of the infertility problems experienced by couples. Azoospermia, having no measurable level of sperm in seminal fluid, is one of the known conditions resulting in male infertility. In order to elucidate the complex molecular mechanisms
Jorid T Stuenaes et al.
British journal of pharmacology, 160(1), 116-129 (2010-04-24)
Genetic approaches have documented protein kinase B (PKB) as a pivotal regulator of heart function. Insulin strongly activates PKB, whereas adrenaline is not considered a major physiological regulator of PKB in heart. In skeletal muscles, however, adrenaline potentiates insulin-stimulated PKB
-
What is the extinction coefficient for this 80 mg/mL protein standard?
1 answer-
Helpful?
-
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
