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I5502

Sigma-Aldrich

Isopropil β-D-1-tiogalattopiranoside

≥99% (TLC)

Sinonimo/i:

IPTG, Isopropil β-D-tiogalattoside

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About This Item

Formula empirica (notazione di Hill):
C9H18O5S
Numero CAS:
367-93-1
Peso molecolare:
238.30
Beilstein:
4631
Numero CE:
206-703-0
Numero MDL:
MFCD00063273
Codice UNSPSC:
12352201
ID PubChem:
24896069
NACRES:
NA.31
Prezzi e disponibilità al momento non sono disponibili

Descrizione

non-ionic

Livello qualitativo

300

Saggio

≥99% (TLC)

Stato

powder

tecniche

thin layer chromatography (TLC): suitable

Impurezze

10.0-22.0% Dioxane

Colore

white to off-white

Punto di fusione

120 -122  °C ((248 - 252 °F ))

Solubilità

water: 50 mg/mL, clear, colorless

Temperatura di conservazione

2-8°C

Stringa SMILE

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
BPHPUYQFMNQIOC-NXRLNHOXSA-N

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Categorie correlate

Applicazioni

L′IPTG viene comunemente utilizzato nelle procedure di clonazione che richiedono l′induzione dell′attività della β-galattosidasi. Viene adoperato insieme a X-Gal o Bluo-Gal nella selezione blu-bianco delle colonie di batteri ricombinanti che inducono l′espressione dell′operone lac nell′Escherichia coli. L′IPTG agisce legandosi al repressore lacI e alterandone la conformazione e impedisce così la repressione del gene che codifica la β-galattosidasi, lacZ.

Altre note

Analogo del galattosio non metabolizzabile.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Ricostituzione

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Avvertenze

WarningDanger

Indicazioni di pericolo

H319 - H351 - H319 - H350

Classi di pericolo

Carc. 1B - Eye Irrit. 2

Rischi supp

EUH019

Codice della classe di stoccaggio

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000398605
0000398606
0000287005
0000369115
0000361359

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Sigma-Aldrich

772135

Phenyl 1-thio-β-D-galactopyranoside

Ampicillina

Sigma-Aldrich

A9518

Ampicillina

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Proceedings of the National Academy of Sciences of the United States of America, 111(27), E2787-E2796 (2014-06-01)
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Killed avian influenza virus (AIV) vaccines have been used to control H5N1 infections in countries where the virus is endemic. Distinguishing vaccinated from naturally infected birds (DIVA) in such situations however, has become a major challenge. Recently, we introduced the
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Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
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Keratins are cytoskeletal intermediate filament proteins that are increasingly being recognised for their diverse cellular functions. Here we report the consequences of germ line inactivation of Keratin 76 (Krt76) in mice. Homozygous disruption of this epidermally expressed gene causes neonatal
Tobias Goldmann et al.
The EMBO journal, 34(12), 1612-1629 (2015-04-22)
Microglia are tissue macrophages of the central nervous system (CNS) that control tissue homeostasis. Microglia dysregulation is thought to be causal for a group of neuropsychiatric, neurodegenerative and neuroinflammatory diseases, called "microgliopathies". However, how the intracellular stimulation machinery in microglia
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Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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