IPTG-RO

Isopropil β-D-1-tiogalattopiranoside

mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)

• Sinonimo/i: IPTG, Isopropil β-D-tiogalattoside
• Formula empirica (notazione di Hill): C₉H₁₈O₅S
• Numero CAS: 367-93-1
• Peso molecolare: 238.30
• PubChem Substance ID: 329815691
• UNSPSC Code: 12352200
• Beilstein/REAXYS Number: 4631
• MDL number: MFCD00063273
• NACRES: NA.54

Proprietà

• SMILES string: CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N
• form: powder
• mol wt: 238.3
• packaging: pkg of 1 g (10724815001), pkg of 5 g (11411446001)
• manufacturer/tradename: Roche
• shipped in: wet ice
• storage temp.: 2-8°C

Descrizione

General description

Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Application

L′IPTG viene comunemente utilizzato nelle procedure di clonazione che richiedono l′induzione dell′attività della β-galattosidasi. Viene adoperato insieme a X-Gal o Bluo-Gal nella selezione blu-bianco delle colonie di batteri ricombinanti che inducono l′espressione dell′operone lac nell′Escherichia coli. L′IPTG agisce legandosi al repressore lacI e alterandone la conformazione e impedisce così la repressione del gene che codifica la β-galattosidasi, lacZ.

Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.

Features and Benefits

Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 10⁹ dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.

Preparation Note

Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long

Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.

Other Notes

Analogo del galattosio non metabolizzabile.

For life science research only. Not for use in diagnostic procedures.

The amount of labeled DNA depends on:

• amount of template DNA
• purity of template DNA
• conformation of template DNA
• average size of labeled fragment

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable