Solutions of Fast Green FCF are used much like solutions of Light Green SF Yellowish. The advantage of using Fast Green FCF is generally that the stain is more intense. The working solution is typically prepared fresh each day or used for a short period of time.
F7258
Fast Green FCF
certified by the Biological Stain Commission
Sinonimo/i:
Food green 3
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25 G
CHF 245.00
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25 G
CHF 245.00
About This Item
Formula condensata:
C37H34N2O10S3Na2
Numero CAS:
Peso molecolare:
808.85
Colour Index:
42053
Beilstein:
5718212
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47
Prodotti consigliati
Grado
certified by the Biological Stain Commission
Livello qualitativo
Stato
powder
Composizione
dye content, ≥90%
Punto di fusione
290 °C (dec.) (lit.)
Solubilità
H2O: 1 mg/mL
applicazioni
diagnostic assay manufacturing
hematology
histology
Temperatura di conservazione
room temp
Stringa SMILE
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
RZSYLLSAWYUBPE-UHFFFAOYSA-L
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Applicazioni
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Compatibilità
Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Muta. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
352.4 °F - closed cup
Punto d’infiammabilità (°C)
178 °C - closed cup
Dispositivi di protezione individuale
Eyeshields, Gloves
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What is the proper method to prepare Fast Green FCF for use as a counterstain?
1 answer-
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Looking for a green dye to add to electrolyzed water. Something biodegradable that will disappear fast on skin.
1 answer-
It is advised to source a FD&C (Food, Drug & Cosmetic) grade dye for use on human skin. Assuming there is suitable electrolyzed water, green food coloring should suffice for the intended purpose. The duration for which the dye remains on the skin would depend on factors such as the pH of the water used and the specific characteristics of the dye, which the customer would need to determine.
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