E2129

Evans Blue

Dye content ≥75 %, Powder

• Sinonimo/i: Direct Blue 53
• Formula empirica (notazione di Hill): C₃₄H₂₄N₆Na₄O₁₄S₄
• Numero CAS: 314-13-6
• Peso molecolare: 960.81
• NACRES: NA.47
• PubChem Substance ID: 24894456
• UNSPSC Code: 12171500
• EC Number: 206-242-5
• MDL number: MFCD00004021
• Colour Index Number: 23860

Proprietà

• Nome del prodotto: Evans Blue, Dye content ≥75 %
• InChI key: ATNOAWAQFYGAOY-GPTZEZBUSA-J
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• form: powder
• composition: Dye content, ≥75%
• technique(s): titration: suitable
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• Quality Level: 200
• Gene Information: human ... PTPN1(5770), PTPRF(5792)

Descrizione

General description

Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.

Application

Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.

Biochem/physiol Actions

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability.[1] It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.[2]

Informazioni sulla sicurezza

• pictograms: GHS08
• signalword: Danger
• hcodes: H350
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable