This product has not be tested for in vivo or in vitro use, but has been used successfully in mouse models by other researchers. Please review the PEER REVIEWED PAPERS above for list of publications. Please see the link below to review an example:
https://pmc.ncbi.nlm.nih.gov/articles/PMC5974208/
F7252
Fast Green FCF
Dye content ≥85 %, powder
Sinonimo/i:
Food green 3
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
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5 G
CHF 63.00
25 G
CHF 237.00
100 G
CHF 663.00
CHF 63.00
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Informazioni su questo articolo
Formula condensata:
C37H34N2O10S3Na2
Numero CAS:
Peso molecolare:
808.85
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
EC Number:
219-091-5
MDL number:
Beilstein/REAXYS Number:
5718212
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Fast Green FCF, Dye content ≥85 %
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
SMILES string
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
form
powder
composition
Dye content, ≥85%
mp
290 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL, clear
Quality Level
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Categorie correlate
1 of 4
Questo articolo | |||
|---|---|---|---|
| solubility H2O: 1 mg/mL, clear | solubility H2O: 1 mg/mL | solubility - | solubility H2O: 1 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form powder | form powder | form - | form powder |
| composition Dye content, ≥85% | composition dye content, ≥90% | composition - | composition Dye content, ≥65% |
| mp 290 °C (dec.) (lit.) | mp 290 °C (dec.) (lit.) | mp 290 °C (dec.) (lit.) | mp 288 °C (dec.) (lit.) |
General description
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2 - STOT SE 3
target_organs
Respiratory system
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 2
flash_point_f
352.4 °F - closed cup
flash_point_c
178 °C - closed cup
ppe
Eyeshields, Gloves
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Articoli
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
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Is Fast Green FCF (F7252) in vivo grade? Our lab wants to use it for retroorbital injections but wasn't sure if it was safe to be injected in live animals.
1 answer-
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Dear Sirs please advise why there is no expiration date in your product F7252 Lot.No. SHBN0187
1 answer-
his product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information, please access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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