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Merck
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Documenti fondamentali

909475

Sigma-Aldrich

5-Ethynyl uridine

≥95%

Sinonimo/i:

5-EU, EU

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5 MG
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5 MG
CHF 91.40

About This Item

Formula empirica (notazione di Hill):
C11H12N2O6
Numero CAS:
Peso molecolare:
268.22
Numero MDL:
Codice UNSPSC:
41106305
NACRES:
NA.22

CHF 91.40


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Saggio

≥95%

Stato

powder

Impiego in reazioni chimiche

reaction type: click chemistry

Temperatura di conservazione

−20°C

Stringa SMILE

[nH]1[c]([n](cc([c]1=O)C#C)[C@@H]2O[C@@H]([C@H]([C@H]2O)O)CO)=O

InChI

1S/C11H12N2O6/c1-2-5-3-13(11(18)12-9(5)17)10-8(16)7(15)6(4-14)19-10/h1,3,6-8,10,14-16H,4H2,(H,12,17,18)/t6-,7-,8-,10-/m1/s1
QCWBIPKYTBFWHH-FDDDBJFASA-N

Applicazioni

This alkyne-bearing metabolic labeling reagent can be used to measure RNA synthesis in proliferating cells. Once fed to cells, this nucleoside is incorporated during active RNA synthesis and can then be detected using a copper-catalyzed click reaction with fluorescent dyes. The cells can then be analysed by fluorescence imaging, flow cytometry or high content imaging analysis.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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We describe a chemical method to detect RNA synthesis in cells, based on the biosynthetic incorporation of the uridine analog 5-ethynyluridine (EU) into newly transcribed RNA, on average once every 35 uridine residues in total RNA. EU-labeled cellular RNA is
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We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs

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