Giemsa solution protocols recommend diluting with either water or a phosphate buffer. Common phosphate buffers are Weise or Sorensen's phosphate buffer. Depending upon personal preference, the pH used can vary from a low pH of 6.0 to a high of 7.2 In North America it is more common to use a Sorensen's phosphate buffer with a pH ranging from 6.8 to 7.2. A suitable catalog number for a pH 7.2 buffer is P3288. In other parts of the world slightly more acidic phosphate buffers are preferred.
48900
Giemsa Stain
Modified Solution
Sinonimo/i:
Azure eosin methylene blue, Giemsa solution
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
41116121
ID PubChem:
NACRES:
NA.25
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Nome del prodotto
Giemsa Stain, Modified Solution, (for the staining (of cellular blood components and blood parasites))
Tipo
(for research use only)
Livello qualitativo
Durata
limited shelf life
tecniche
microbe id | staining: suitable
Compatibilità
Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes
Stringa SMILE
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
NALREUIWICQLPS-UHFFFAOYSA-N
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Applicazioni
Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
Avvertenza
store at 20°C
Principio
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Stato fisico
Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)
Ricostituzione
Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Organi bersaglio
Eyes
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
53.6 °F
Punto d’infiammabilità (°C)
12 °C
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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WITH WHICH BUFFER SHOULD I DILUTE GIEMSA SOLUTION
1 answer-
Helpful?
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Hi does any lab use this stain for microscopy ( haematology) ? How is it working for you on fresh peripheral blood films ?
1 answer-
Giemsa solution is commonly used for hematology, histology, and parasitology labs. Peripheral blood smears and bone marrow smears are routinely stained in hematology labs. It is always preferred to use fresh peripheral blood smears or bone marrow smears when possible. The colors are more vibrant when the peripheral blood smears are stained fresh.
Helpful?
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Is there a white paper available for buffer selection for Giemsa staining?
1 answer-
Generally, no white papers are available. Giemsa formulations vary in pH, typically ranging between pH 6.4 to 6.9. Quality standards for stained slides differ across institutions and individuals, with varying preferences for pH buffers.
Helpful?
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