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318752

Sigma-Aldrich

Bromothymol Blue sodium salt solution

0.04 wt. % in H2O

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100 ML
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100 ML
CHF 35.90

About This Item

Formula empirica (notazione di Hill):
C27H27Br2NaO5S
Numero CAS:
Peso molecolare:
646.36
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

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Stato

liquid

Livello qualitativo

Concentrazione

0.04 wt. % in H2O

pH

6.0-7.6, yellow to blue

Densità

0.99 g/mL at 25 °C (lit.)

λmax

615 nm

ε (coefficiente d’estinzione)

≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
NMKFVGALBGZKGW-FKWCIMQXSA-M

Descrizione generale

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Applicazioni

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Azioni biochim/fisiol

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.[1]

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Questions

  1. Do you provide dyes or other solutions used for validating adequate cleaning of equipment?

    1 answer
    1. If you are checking for the adequate cleaning of equipment, there are various ways to do so. pH indicators such as bromothymol blue can be used to ensure that alkaline detergents have been washed away. However, dyes are generally not commonly used to check for sterility or whether a surface is clean of all substances. The use of other dyes may vary, and it's essential to consider the specific use and characteristics of each dye for different applications.

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