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11427598910

Roche

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

Sinonimo/i:

Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate

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About This Item

Formula empirica (notazione di Hill):
C32H52N7O17P3S
Numero CAS:
188755-42-2
Peso molecolare:
931.78
Numero MDL:
MFCD32671794
Codice UNSPSC:
41116100
Prezzi e disponibilità al momento non sono disponibili

Livello qualitativo

100

Saggio

≥98% (HPLC)

Stato

solution

PM

955.5 (biotin-16-ddUTP-Li4)

Confezionamento

pkg of 25 μL (25 nmol; 1mM)

Produttore/marchio commerciale

Roche

Concentrazione

1 mM

tecniche

hybridization: suitable

Temperatura di conservazione

−20°C

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Descrizione generale

Biotin-16-ddUTP, tetralithium salt; 1mM

Applicazioni

  • Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
  • DNA polymerase I (Holoenzyme and Klenow fragment)
  • T4 DNA polymerase
  • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.[1][2]
  • The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique.[3] It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.[4]

Caratteristiche e vantaggi

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Qualità

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

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Certificati d'analisi (COA)

Lot/Batch Number
74115521
27782224
27782223
27782222
27782221

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