11388908910
Roche
Biotin-16-UTP
pkg of 25 μL (250 nmol; 10mM)
Sinonimo/i:
Biotin-16-UTP, biotin
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About This Item
Codice UNSPSC:
41116100
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Saggio
97.7% (HPLC)
Livello qualitativo
Stato
solution
PM
Mr 987.5 (biotin-16-UTP-Li4)
Confezionamento
pkg of 25 μL (250 nmol; 10mM)
Produttore/marchio commerciale
Roche
Colore
colorless
Solubilità
water: miscible
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Descrizione generale
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Applicazioni
Biotin-labeled RNA can be used as a hybridization probe for:
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Qualità
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Stato fisico
Biotin-16-UTP, tetralithium salt, 10mM solution
Altre note
For life science research only. Not for use in diagnostic procedures.
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
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