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11426338910

Roche

Anti-Fluorescein-AP, Fab fragments

from sheep

Sinonimo/i:

antibody

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About This Item

Codice UNSPSC:
12352203

Origine biologica

sheep

Livello qualitativo

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

solution

Confezionamento

pkg of 150 U (200 μl)

Produttore/marchio commerciale

Roche

Isotipo

IgG

Temperatura di conservazione

2-8°C

Descrizione generale

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

Specificità

The polyclonal antibody reacts with free and bound fluorescein.

Applicazioni

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Southern blot
  • Western blot
  • Electrochemical biosensing technique for the detection of 16S rRNA

Azioni biochim/fisiol

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

Caratteristiche e vantaggi

Contents
Solution, stabilized

Nota sulla preparazione

  • Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
  • ELISA: 150 to 300mU/ml
  • Immunohistocytochemistry: 250 to 500mU/ml
  • In situ hybridization: 1.5 to 7.5U/ml
  • Southern blot: 150mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Altre note

For life science research only. Not for use in diagnostic procedures.

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Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Skin Sens. 1

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


Certificati d'analisi (COA)

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