11214667001

Anti-Digoxigenin, Fab fragments

from sheep

• Sinonimo/i: anti-digoxigenin, digoxigenin
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: sheep
• Livello qualitativo: 100
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: lyophilized
• Confezionamento: pkg of 1 mg
• Produttore/marchio commerciale: Roche
• tecniche: ELISA: 2-4 μg/mL; immunohistochemistry: 0.5-1 μg/mL; western blot: 0.5-2 μg/mL
• Isotipo: IgG1
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.[1]

Specificità

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Applicazioni

Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:

• ELISA
• Immunohistocytochemistry[2][3]
• In situ hybridization
• Western blot
• Flow cell construction.[4][5][6]

Caratteristiche e vantaggi

Specific to digoxigenin and digoxin.

Sequenza

The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.

Nota sulla preparazione

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 1 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml

Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!

Ricostituzione

Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.

Risultati analitici

Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash