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Documenti fondamentali

11426320001

Roche

Anti-Fluorescein

from mouse IgG1 (clone B13-DE1)

Sinonimo/i:

antibody

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About This Item

Codice UNSPSC:
12352200

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

B13-DE1, monoclonal

Stato

lyophilized

Confezionamento

pkg of 100 μg

Produttore/marchio commerciale

Roche

Isotipo

IgG1

Temperatura di conservazione

2-8°C

Descrizione generale

Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.

Contents
Lyophilizate, stabilized

Specificità

The monoclonal antibody reacts with free and bound fluorescein.

Applicazioni

Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
Anti-Fluorescein has been used to capture amplicon in quadruple-tagging PCR and nucleic acid lateral flow assay for simultaneous detection of foodborne pathogens.

Nota sulla preparazione

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.

Ricostituzione

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.

Altre note

For life science research only. Not for use in diagnostic procedures.

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Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 3 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


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