03359123001

Alkaline Phosphatase recombinant, highly active

EIA Grade, from Pichia Pastoris

• Classificazione EC (Enzyme Commission): 3.1.3.1 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204
• NACRES: NA.21

Proprietà

• Livello qualitativo: 100
• Saggio: ≥95% (HPLC)
• Stato: buffered aqueous solution (ready-to-use, 20 mg/ml)
• Attività specifica: ≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
• Confezionamento: pkg of 10 mg (500 μl)
• Produttore/marchio commerciale: Roche
• Concentrazione: 20 mg/mL (ready-to-use)
• pH ottimale: 8.0(Stability); 9.8(Activity)
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa.[1][2] The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.[3]

The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.

Specificità

Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.

Applicazioni

Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.

Azioni biochim/fisiol

Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids.[2] It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.[4]

Specifiche

≥7,000 U/mg (+37°C; pNPP; DEA)

Stato fisico

Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.

Nota sulla preparazione

Activator: Mg^2-, Co^2- and Mn^2-ions

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): No data available
• Punto d’infiammabilità (°C): No data available