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To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Sinonimo/i:
Alkaline phosphatase-Agarose
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50 UNITS
CHF 281.00
100 UNITS
CHF 482.00
250 UNITS
CHF 1’040.00
1000 UNITS
CHF 3’230.00
CHF 281.00
Spedizione prevista il29 maggio 2025
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Cambia visualizzazione
50 UNITS
CHF 281.00
100 UNITS
CHF 482.00
250 UNITS
CHF 1’040.00
1000 UNITS
CHF 3’230.00
About This Item
CHF 281.00
Spedizione prevista il29 maggio 2025
Prodotti consigliati
Origine biologica
bovine (calf) intestine
Stato
ammonium sulfate suspension
Attività specifica
250-1000 units/mL packed gel
Matrice
beaded agarose
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
Applicazioni
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases. [4]
Azioni biochim/fisiol
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Confezionamento
Package size based on DEA units
Definizione di unità
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
Stato fisico
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
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1. What is the recommended speed for spinning down the phosphatase, alkaline-agarose when using this product?
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