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P4439

Phosphatase, Alkaline from porcine kidney

lyophilized powder, ≥100 DEA units/mg protein

Sinonimo/i:

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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A voi/SKUDisponibilitàPrezzo
100 units
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CHF 63.80

Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Numero CE:
Specific activity:
≥100 DEA units/mg protein

CHF 63.80


Per conoscere la disponibilità, visualizza il carrello

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form

lyophilized powder

Quality Segment

specific activity

≥100 DEA units/mg protein

composition

Protein, ≥50%

storage temp.

−20°C

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling[1].

Biochem/physiol Actions

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

Packaging

Package size based on DEA units

Physical form

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

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Questo articolo
P7640P0114A2356
specific activity

≥100 DEA units/mg protein

specific activity

≥10 DEA units/mg solid

specific activity

≥5,700 DEA units/mg protein

specific activity

≥5,500 DEA units/mg protein

form

lyophilized powder

form

lyophilized powder

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥50%

composition

-

composition

-

composition

-


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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