WB59
Western-Re-Probe Reagent
This Western-Re-Probe Reagent is validated for use in Immunoblotting.
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About This Item
Codice UNSPSC:
41116133
NACRES:
NA.77
Prodotti consigliati
Livello qualitativo
Forma fisica
liquid
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
Designed to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
This Western-Re-Probe Reagent is validated for use in Immunoblotting.
Western-Re-Probe Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.
Applicazioni
Immunoblotting (see comments)
Componenti
5X Re-Probe solution (125 ml)
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
Supplied as a 5X solution.
Altre note
Sufficient volume to strip 25-30 standard blots.
ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes
PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe in water.
2- Incubate the membrane in 1X Western-Re-Probe buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.
Western-Re-Probe Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.
PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetection
ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes
PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe in water.
2- Incubate the membrane in 1X Western-Re-Probe buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.
Western-Re-Probe Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.
PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetection
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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