The low or no protein recovery issue may be attributed to over-concentration of the protein, leading to precipitation. The User Guide provides guidance on maximizing sample recovery: Low sample recovery in the concentrate may be due to adsorptive losses, over-concentration, or passage of sample through the membrane. Adsorptive losses depend upon solute concentration, its hydrophobic nature, temperature and time of contact with filter device surfaces, sample composition, and pH. To minimize losses, remove concentrated samples immediately after centrifugal spin. If the starting sample concentration is high, monitor the centrifugation process in order to avoid over-concentration of the sample. Over-concentration can lead to precipitation and potential sample loss. If the sample appears to be passing through the membrane, choose a lower MWCO Amicon device.
UFC8003
Filtri centrifughi Amicon® Ultra, MWCO 3 kDa
sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa
Sinonimo/i:
Concentratore centrifugo, MWCO di 3 kDa, volume del campione 4 mL
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About This Item
Prodotti consigliati
Materiali
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Livello qualitativo
Sterilità
non-sterile
Nome Commerciale
Amicon®
Confezionamento
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
Produttore/marchio commerciale
Amicon® Ultra
sustainability
Greener Alternative Product
Parametri
4 mL sample volume
tecniche
protein extraction: suitable
protein purification: suitable
Lunghezza
124 mm (4.88 in.)
Diametro
17.3 mm
Superficie filtrante
3 cm2
Volume
4 mL
Volume d’esercizio
≤4 mL
4 mL
Dimensione pori
3 kDa MWCO
Categoria alternativa più verde
Condizioni di spedizione
ambient
Descrizione generale
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. ®
Applicazioni
- ®
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Caratteristiche e vantaggi
Stato fisico
Note legali
Certificati d'analisi (COA)
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Articoli
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Protocolli
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Il proteoma del plasma umano costituisce una vasta risorsa per la scoperta e l’investigazione di biomarcatori.
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What could be the reason for no protein recovery (in the concentrate or flow through) while using the UFC800324 to purify protein? Could the protein may be absorbed in the filter?
1 answer-
Helpful?
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What is max g force these filters can take? i.e. what sort of rpm or g force should I spin at?
1 answer-
The UFC8003 protocol states to spin at 7,500 × g maximum for approximately 10–40 minutes. See the image below for further details.
Helpful?
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Can we centrifuge these tubes in a normal 15 mL swinging bucket centrifuge?
1 answer-
Yes, the device can be used in centrifuges with a swinging bucket or fixed-angle rotor.
Helpful?
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