There is no maximum protein load for these devices, and optimal protein concentrations must be determined empirically. We do recommend a clarifying spin prior to filtration to bring down larger particulates.
UFC8100
Filtri centrifughi Amicon® Ultra, MWCO 100 kDa
sample volume 4 mL, regenerated cellulose membrane, MWCO 100 kDa
Sinonimo/i:
Concentratore centrifugo, MWCO di 100 kDa, volume del campione 4 mL
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8 PKG
CHF 152.00
24 PKG
CHF 385.00
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CHF 1’090.00
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Cambia visualizzazione
8 PKG
CHF 152.00
24 PKG
CHF 385.00
96 PKG
CHF 1’090.00
About This Item
Codice UNSPSC:
41104916
eCl@ss:
36100101
NACRES:
NB.24
Prodotti consigliati
Materiali
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Livello qualitativo
Sterilità
non-sterile
Nome Commerciale
Amicon®
Confezionamento
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
Produttore/marchio commerciale
Amicon® Ultra
sustainability
Greener Alternative Product
Parametri
4 mL sample volume
tecniche
protein extraction: suitable
protein purification: suitable
Lunghezza
124 mm (4.88 in.)
Diametro
17.3 mm
Superficie filtrante
3 cm2
Volume
4 mL
Volume d’esercizio
≤4 mL
4 mL
Dimensione pori
100 kDa MWCO
Categoria alternativa più verde
Condizioni di spedizione
ambient
Descrizione generale
®® >
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. ®
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. ®
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Applicazioni
- ®
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Caratteristiche e vantaggi
- ®
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Note legali
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
10-13 - German Storage Class 10 to 13
Certificati d'analisi (COA)
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Protocolli
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Il proteoma del plasma umano costituisce una vasta risorsa per la scoperta e l’investigazione di biomarcatori.
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What is the maximum recommended starting protein concentration from Vero cell lysate to avoid overwhelming the filter?
1 answer-
Helpful?
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Can I use Amicon Ultra 100 kDa filter only for protein sample filtration, to avoid concentration or sample purification?
1 answer-
This device is intended for use as a protein concentrator. Proteins above 100 kDa will be retained while smaller proteins will pass through to the filtrate fraction. Other particles within the sample may be present in both the filtrate and the retentate, depending on the nature of the particle, the nature of the filter membrane, and the centrifugation protocol or technique used. To discuss this further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
Helpful?
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Les Amicons sont-il RNase/DNase free- DNA/RNA free? Traitement à l'oxyde d'éthylène?
1 answer-
The Amicon products are not tested for RNase/DNase activity. These items are not considered RNase/DNase free. They are not ethylene oxide treated.
Helpful?
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