ABS1506

Anti-phospho-SIN1 (Thr398)

from rabbit, purified by affinity chromatography

• Sinonimo/i: Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, rat
• Confezionamento: antibody small pack of 25 μL
• tecniche: inhibition assay: suitable (peptide); western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_001006618.1
• N° accesso UniProt: Q9BPZ7
• modifica post-traduzionali bersaglio: phosphorylation (pThr398)
• Informazioni sul gene: human ... MAPKAP1(79109)

Descrizione

Descrizione generale

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt: Q9BPZ7; also known as TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1) is encoded by the MAPKAP1 (also known as MIP1, SIN1) gene (Gene ID: 79109) in human. mSIN1 is a subunit of mTORC2 regulates cell growth and survival in response to hormonal signals. It is ubiquitously expressed, with highest levels in heart and skeletal muscle. Six isoforms of SIN1 have been described that are produced by alternative splicing. All isoforms with the exception of isoform 4 can be incorporated into the mTORC2 complex. mTORC2 is shown to be activated by growth factors, but in contrast to mTORC1, it is nutrient-insensitive. mTORC2 phosphorylates serine 473 in Akt1, which facilitates the phosphorylation of Threonine 308 by PDK1, thereby allowing full activation of Akt. Genetic knockout of SIN1 is reported to abolish serine 473 phosphorylation of Akt and disrupts the RICTOR-mTOR interaction. mTORC2 also regulates the phosphorylation of SGK1 at serine 422. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and auto-phosphorylation.

Specificità

This rabbit polyclonal antibody detects human Stress-activated MAP kinase-interacting protein 1 phosphorylated on Threonine 398.

Immunogeno

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphorylated Threonine 398 from human Stress-activated MAP kinase-interacting protein 1 (SIN1).

Applicazioni

Anti-phospho-SIN1 (Thr398), Cat. No. ABS1506, is a rabbit polyclonal antibody that detects Target of rapamycin complex 2 subunit MAPKAP1 (SIN1) and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with L6 cells treated with IGF-1 for peptide block analysis.

Research Category
Signaling

Qualità

Evaluated by Western Blotting in L6 cells treated with IGF-1.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-SIN1 (Thr398) in lysate from L6 cells treated with IGF-1.

Descrizione del bersaglio

~65 kDa observed; 59.12 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable