234109-M

Colcemid

Cell synchronization agent.

• Sinonimo/i: Colcemid, Demecolcine
• Formula empirica (notazione di Hill): C₂₁H₂₅NO₅
• Numero CAS: 477-30-5
• Peso molecolare: 371.43
• Numero MDL: MFCD00075459
• Codice UNSPSC: 12352200

Proprietà

• Saggio: ≥98% (HPLC)
• Forma fisica: crystalline powder
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Solubilità: chloroform: 10 mg/mL; ethanol: soluble
• Temperatura di conservazione: 10-30°C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3; NNJPGOLRFBJNIW-UHFFFAOYSA-N

Descrizione

Descrizione generale

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Azioni biochim/fisiol

Primary Target
Depolymerizes microtubules and limits microtubule formation.

Attenzione

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

Altre note

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany