207000

Calcineurin Autoinhibitory Peptide

The Calcineurin Autoinhibitory Peptide controls the biological activity of Mn2+-stimulated calcineurin activity. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Sinonimo/i: Calcineurin Autoinhibitory Peptide, ITSFEEAKGLDRINERMPPRRDAMP
• Formula empirica (notazione di Hill): C₁₂₄H₂₀₅N₃₉O₃₉S₂
• Peso molecolare: 2930.32
• Codice UNSPSC: 12352202

Proprietà

• Livello qualitativo: 100
• Saggio: ≥97% (HPLC)
• Forma fisica: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Solubilità: water: soluble
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Corresponds to a C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin. Specific calcineurin inhibitor. Does not inhibit CaM kinase II, protein phosphatase I or 2A. Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated activity.

Specific calcineurin inhibitor. Corresponds to the C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin (PP2B). Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P-myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated enzyme activity. Does not inhibit CaM kinase II, protein phosphatase 1 or 2A.

Azioni biochim/fisiol

Cell permeable: no

Primary Target
Mn²⁺-stimulated calcineurin activity

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 10 µM using 32P-myosin light chain as a substrate

Confezionamento

Yes

Attenzione

Toxicity: Standard Handling (A)

Sequenza

H-Ile-Thr-Ser-Phe-Glu-Glu-Ala-Lys-Gly-Leu-Asp-Arg-Ile-Asn-Glu-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-OH

Stato fisico

Supplied as a trifluoroacetate salt.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions are stable for up to 2 months at -20°C.

Altre note

Perrino, B.A., et al. 1995. J. Biol. Chem. 270, 340.
Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett.337, 128.
Yokoyama, N., et al. 1993. Arch. Biochem. Biophys.300, 615.
Matsui, H., et al. 1991. Biochem. Int.24, 1119.
Hashimoto, Y., et al. 1990. J. Biol. Chem.265, 1924.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable