14-930-M

IRE1, active

• Codice UNSPSC: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• PM: M_w 62 kDa
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable (kinase)
• N° accesso NCBI: NM_001433
• N° accesso UniProt: O75460

Descrizione

Descrizione generale

Product Source: N-terminal 6His-tagged, recombinant, human IRE1 amino acids 465-end expressed by baculovirus in Sf21 insect cells.

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Confezionamento

Also available in 250μg size as catalog # 14-930M and in bulk as catalog # 14-930-K.

Qualità

Routinely evaluated by phosphorylation of MBP.

Stato fisico

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Stoccaggio e stabilità

6 months at -70°C

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable