05-449-C
Anti-Bin1, clone 99D (Ascites Free) Antibody
clone 99D, 1 mg/mL, from mouse
Sinonimo/i:
Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, Bin1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
99D, monoclonal
Reattività contro le specie
mouse, chicken, human
Concentrazione
1 mg/mL
tecniche
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2bκ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
chicken ... Bin1(424228)
human ... BIN1(274)
mouse ... Bin1(30948)
Categorie correlate
Descrizione generale
Bin1, also known as Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, and encoded by the gene BIN1/AMPHL, is another protein involved in the regulation of synaptic vesicle endocytosis and interestingly also acts as tumor suppressor and inhibits malignant cell transformation. BIN1 is expressed in many tissues but has the highest expression in brain and muscle. BIN1 has isoforms and particular isoforms are expressed in particular tissues. For instance BIN1 isoform 11A is only expressed in the brain and found in axon initial segments and nodes of Ranvier. Mutations in BIN1 are associated with a disordered called centronuclear 2 myopathy. BIN1 is localized to nucleus but some isoforms are associated with cytoplasm.
Immunogeno
Linear peptide corresponding to human Bin1.
Applicazioni
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Bin1 in human cerebellum and human skeletal muscle myocytes tissue.
Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.
Qualità
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
Descrizione del bersaglio
~60 kDa observed
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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