SIRNA
MISSION® siRNA Product Offerings
Custom and Predesigned siRNA
Sinonimo/i:
Custom siRNA, High-throughput siRNA, Predesigned siRNA, siRNA, siRNA pools
About This Item
Prodotti consigliati
PREDESIGNED & Custom esiRNA
Biologically-prepared, esiRNA are comprised of a heterogeneous pool of siRNA (natural RNA, no modifications) that all target the same mRNA sequence.
- Format: Individual esiRNA, panels, and libraries available. Individual: 20 or 50 µg lyophilized in tubes. Panels & libraries: 1, 2.5, or 5 µg in solution in plates
- Purification: Q-Sepharose separation, isopropanol precipitation & ethanol washing
- Sequence Form: Pool of hundreds of siRNA with an average duplex length of 21 bp
- Quality Control: PCR product of cDNA clone is analyzed by gel electrophoresis & DNA sequencing; digestion reaction is analyzed by gel electrophoresis
- Learn more
Predesigned
Custom Request
Predesigned siRNA
Extensive collection of human, mouse and rat siRNA. Multiple sequences are available for every gene, with rankings to indicate performance.
- Scale: 10, 25, 50, and 250 nmol
- Purification: Desalt or HPLC
- Sequence Length: 21 bases
- Modifications: Not available for this item
- Quality Control: Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.
- Format: Supplied dry
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Custom siRNA
Custom siRNA sequences with a wide range of modifications, purifications and quantities to tailor your unique sequence.
- Scale: 10, 25, 50, and 250 nmol
- Purification: Desalt or HPLC
- Sequence Lengths: 19 - 27 bases
- Modifications: Comprehensive offering of modifications and dyes (2'-O-Methyl, Amine, Cyanine dyes, 6-FAM, Biotin, Phosphate, Thiol, Abasic, Phosphorothioate linkages, 2'-Fluoro).
- Quality Control: Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.
- For additional purifications, sequence length or modifications,
email [email protected] - Custom siRNA sequence design available for a variety of species,
Learn more
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Plates
Fast siRNA
Expedite your siRNA order! Orders are prioritized in the production process with a turn-around-time of only 3 days.
- FAST siRNA is delivered in solution at concentrations of 50 µM (2 OD) or 100 µM (5 OD).
- US orders must be received by 11 AM CST. European orders must be received by 11 AM CET.
- Easy to use Fast siRNA ordering tool.
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iScale Oligos™ - siRNA
siRNA provided in larger quantities for in vivo, high throughput or diagnostic projects.
- Amount: 1 – 10 mg, 50 mg – 1 g
- Purification: Desalt, RPC (cartridge purified), HPLC and In vivo quality
- Sequence Length: 19 - 45 bases
- Comprehensive offering of modifications including dyes, amines, biotin, linkers, etc.
- Quality Control: Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.
- In vivo option includes HPLC-purification to >/= 90%, desalt, filtration and endotoxin testing.
- To inquire for availability of additional quantities, modifications or purifications, email [email protected]
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siRNA Controls
Universal Negative Controls
- Two distinct siRNA control sequences designed for use in Human, Mouse, or Rat cells.
- Validated with Agilent 40K human gene arrays to ensure no significant nonspecific gene interactions.
- Functionally validated to show no immune response via qRT PCR.
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Positive Control siRNA.
- Validated human and rat positive controls to simplify transfection optimization.
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Custom miRNA
Custom miRNA sequences with a wide range of modifications, purifications, and quantities to tailor your unique sequence.
- Amount: 2 OD (10 nmol), 5 OD (25 nmol), 10 OD (50 nmol) & 50 OD (250 nmol)
- Purification: Desalt or HPLC
- Length: 19-27mer duplexes
- Modifications: Biotin, Phosphate, Amino-Modifier C7, 6-FAM, Cyanine 3, Cyanine 5, Cyanine 5.5, 2’-OMe, Phosphorothioate, Thiol-Modifier C6 S-S and Thiol-Modifier C3
- Quality Control: Individual strands are analyzed by mass spectrometry. Depending on the manufacturing site, PAGE may be used to assess miRNA duplexes. miRNA quantity is systematically validated by UV absorbance at 260 nm.
- For additional modifications and purification requests, email [email protected]
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RNA Oligos
- Scale: 0.05, 0.2, 1.0 µmol
- Purification: Desalt, HPLC and PAGE
- Sequence Length: 7 - 45 bases
- Modification options available
- Quality Control: 100% Mass Spectrometry
- Format: Supplied dry
- Learn more about RNA Oligos
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MISSION® siRNA Transfection Reagent
- Superior gene silencing in a wide variety of eukaryotic cell types
- For use with siRNA, esiRNA, miRNA mimics and Synthetic miRNA inhibitors
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Articoli
Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments.
Learn about precision Sigma-Aldrich Molecular Beacon oligonucleotide probes used for sequence detection in qPCR for in vitro research.
Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Contenuto correlato
We can quickly synthesize and ship custom siRNA using your sequences.
RNA Oligo Specifications, Shipping Schedule, Minimum Yield Guarantees, Quality Control, Quality Assurance
Per chi commercializza kit di diagnostica molecolare (MDx), prepara test sviluppati in laboratorio (LDT) o conduce studi preclinici sugli agenti terapeutici, il livello qualitativo del kit, del saggio o dell’agente terapeutico finale deve essere eccezionale per soddisfare le severe normative o i rigorosi standard pertinenti.
As the world’s leading supplier of oligonucleotides, Sigma-Aldrich delivers the largest portfolio of expertise to its diagnostic partners. ISO 13485 & cGMP/ASR certified.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
