SRP3283

FETUIN A human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonyme(s) : Alpha-2-HS-glycoprotein
• Numéro CAS: 9014-81-7
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Essai: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≥100 nM
• Poids mol.: 45.0-55.0 kDa
• Conditionnement: pkg of 50 μg
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: P02765
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... AHSG(197)

Description

Description générale

The gene AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is mapped to human chromosome 3q27.[1] Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors.[2][3] Fetuin A is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages.[4] The protein has cystatin-like domains, a calcium phosphate-binding site and a TGF (transforming growth factor)-β cytokine-binding motif.[1] Recombinant human fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant fetuin-A migrates at an apparent molecular weight of 45–55kDa by SDS-PAGE run under nonreducing conditions.

Application

FETUIN A human has been used to stimulate mRNA expression in human monocytic THP1 cells, human in vitro differentiated adipocytes and C57BL/6 mice.[5]

Actions biochimiques/physiologiques

AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells.[2] It also can act as a natural antagonist against specific TGF-β (transforming growth factor β) and BMP (bone morphogenetic protein) signaling proteins.[1] High levels of fetuin-A in the serum is associated with mild osteogenesis imperfecta. On the other hand, reduced levels are seen in Paget′s disease.[1] It is predicted as a potential biomarker for the early diagnosis of hypopharyngeal squamous cell carcinoma.[6]

Forme physique

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable