H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

• Synonyme(s) : Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• Numéro CAS: 37326-33-3
• Numéro de classification (Commission des enzymes): 3.2.1.35 (BRENDA, IUBMB)
• Numéro CE : 253-464-3
• Numéro MDL: MFCD00131351
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Conjugué: conjugate (Glucosaminoglycan)
• Niveau de qualité: 200
• Type: Type II
• Forme: lyophilized powder
• Activité spécifique: ≥300 units/mg solid
• Poids mol.: 55 kDa
• Température de stockage: −20°C

Description

Description générale

Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[1] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[2]

Application

Hyaluronidase from sheep testes has been used:

• in flow cytometry[3]
• in the isolation of putative spermatogonial stem cells (SSCs)[4]
• to study the effects of magnetic stimulation in magnetic hydrogel properties[5]
• in immunohistochemistry to visualize collagen type II distribution[6][7]
• to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[8]
• for the degeneration of hydrogels[9]

Actions biochimiques/physiologiques

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Définition de l'unité

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Forme physique

Lyophilized powder containing lactose

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)