L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
G2150
L-glutamine solution
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
Synonyme(s) :
L-Glutamine for cell culture
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20 ML
31.70 CHF
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20 ML
31.70 CHF
About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352209
eCl@ss :
32160406
ID de substance PubChem :
Nomenclature NACRES :
NA.75
Produits recommandés
Qualité
Hybri-Max™
Niveau de qualité
Stérilité
sterile-filtered
Essai
≥99% (TLC)
Forme
solution
Technique(s)
cell culture | hybridoma: suitable
Impuretés
endotoxin, tested
Couleur
clear colorless
Conditions d'expédition
dry ice
Température de stockage
−20°C
Chaîne SMILES
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
Clé InChI
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Informations sur le gène
rat ... Ggt1(116568)
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Catégories apparentées
Description générale
La L-glutamine est un acide aminé essentiel qui sert d'importante source d'énergie aux cellules en culture et constitue un composant crucial des milieux de culture. La L-glutamine est très stable sous forme de poudre sèche ou de solution congelée. Elle se dégrade toutefois assez rapidement dans les milieux liquides et les solutions mères. L'atteinte de performances cellulaires optimales nécessite généralement l'ajout, au milieu, d'un supplément en L-glutamine avant utilisation.
Application
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Notes préparatoires
Prepared in cell culture grade water.
Informations légales
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Application
Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Articles
Importance and uses of glutamine in hybridoma and mammalian cell culture
Chromatograms
application for HPLC-
Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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